Coding

Part:BBa_K4231015:Design

Designed by: yang qingyan   Group: iGEM22_USTC   (2022-09-30)


Borneol diphosphate synthase


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal SpeI site found at 931
    Illegal PstI site found at 748
    Illegal PstI site found at 1534
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal SpeI site found at 931
    Illegal PstI site found at 748
    Illegal PstI site found at 1534
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 275
    Illegal BglII site found at 407
    Illegal XhoI site found at 536
    Illegal XhoI site found at 692
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal SpeI site found at 931
    Illegal PstI site found at 748
    Illegal PstI site found at 1534
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal SpeI site found at 931
    Illegal PstI site found at 748
    Illegal PstI site found at 1534
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 1429


Design Notes

codon optimization before applied to yeast cells


Source

genome of Blumea balsamifera.

References